http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2017093319-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c1755eede76ea7e719a3e0d2843cd793 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2015-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6759de19f29f2b6021fa5d6cafc6fb50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_11231e2315eddd8b3f3620ea0f1f4207 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43afc2837b782da429adb25dc08d08eb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dcee8eda9978db3bc91706626b9aceef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cdcbcbf6541784ec2ccbb8013c06835e |
| publicationDate | 2017-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2017093319-A |
| titleOfInvention | Nucleic acid amplification method |
| abstract | The present invention provides a technique related to PCR in which nucleic acid amplification is reliably performed at higher speed. A method for amplifying a nucleic acid by polymerase chain reaction, wherein the surface temperature of a container containing a sample containing a target nucleic acid and a primer is increased to a predetermined first target temperature T11 (° C.) or higher. Including an overheating step of lowering the temperature to T11 or lower, wherein T11 is 90 to 105 ° C., and the highest temperature T10 (° C.) in the overheating step is 5 to 30 ° C. higher than T11, and from T10 to The time required for lowering the temperature to T11 is 1 to 10 seconds, the predetermined temperature raising and lowering cycle including the overheating step is repeated a plurality of times, and the required time per one temperature raising and lowering cycle is 35 seconds or less Is provided. [Selection] Figure 1 |
| priorityDate | 2015-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 20.