| abstract |
[Problem] To provide a method for detecting a target cell in a biological sample, which is carried out at a low cost and at a low cost with respect to circulating tumor cells (CTC) which are deeply related to cancer prognosis and therapeutic success. The method includes the following steps (1) to (3). (1) Steps (i) to (iii) for preparing the following substances: (i) a nucleic acid complex including a template nucleic acid, and a signal-generating molecule-modified nucleic acid probe and a signal-suppressing molecule-modified nucleic acid probe; (ii) a target A solution containing a nucleic acid aptamer complex comprising a nucleic acid aptamer that specifically binds to a cell surface material of the cell and a trigger nucleic acid, and (iii) a fuel nucleic acid, (2) a cell that is captured on a carrier, Step (1) Nucleic acid aptamer complex, nucleic acid complex, and fuel nucleic acid addition step (3) Signal detection step [selection figure] None |