http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016538264-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-24 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-18 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 |
| filingDate | 2014-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2016538264-A |
| titleOfInvention | Purification method of recombinant protein |
| abstract | A method is provided for purifying a recombinant protein from a mixture comprising the recombinant protein and other related proteins. Binding a recombinant protein to an ion exchange medium using a first equilibration buffer having a first conductivity and a first pH, a second equilibration buffer having a second conductivity and a second pH. Equilibrating the ion exchange medium to which the protein is bound using the liquid, washing the ion exchange medium with the washing liquid having the third electrical conductivity and gradually increasing pH, and the first related protein from the ion exchange medium Eluting the target recombinant protein from the ion exchange medium by rinsing the ion exchange medium with a first eluate having a fourth electrical conductivity and a fourth pH, and having a fifth electrical conductivity and a fifth pH Subsequently washing off the ion exchange medium with the second eluate to elute the second related protein from the ion exchange medium. [Selection figure] None |
| priorityDate | 2013-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 39.