| abstract |
The present invention is an in vitro method for producing isolated mammalian primitive retinal stem cells (pRSCs), comprising: (a) cells that do not contain feeder cells, feeder conditioned media or serum Culturing isolated embryonic stem cells (ESCs) from mammals in a culture medium to produce and proliferate a culture of isolated ESCs; and (b) so proliferated A culture of isolated ESCs is contacted with one or more of Wnt or an inhibitor of TGF-β / BMP signaling, and the isolated ESCs of (a) are differentiated into primitive retinal stem cells, thereby isolated mammals A method comprising producing pRSCs. [Selection] Figure 1 |