http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016525878-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07049 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1276 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 2014-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-09-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2016525878-A |
| titleOfInvention | HIV type 1 group O reverse transcriptase active at high temperature |
| abstract | The present invention is in the field of biotechnology. In particular, the present invention is expressed and purified in bacteria and at various positions in the amino acid sequence of the human immunodeficiency virus type 1 (HIV-1) group O reverse transcriptase. It relates to a modified reverse transcriptase. This reverse transcriptase was modified at positions 358, 359 and 360, and also a variant of this enzyme with changes at positions 355 and 357, or 478, or 69 (in this case 2 There are two amino acid insertions). These polymerases have higher activity than non-mutant enzymes at high temperatures (60 ° C. or higher). Furthermore, the DNA synthesis ability is maintained at a temperature of 70 ° C. or higher. Furthermore, the copy fidelity of these enzymes is not significantly different from that of the non-mutated reverse transcriptase. [Selection figure] None |
| priorityDate | 2013-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 744.