| abstract |
Disclosed herein are compositions for inactivating the human TCRα gene, including modified LAGLIDADG homing endonuclease (LHE) and its derivatives, particularly those derived from members of the I-OnuI subfamily of LHE. Also provided are polynucleotides encoding the above-mentioned endonucleases, vectors containing the polynucleotides, cells containing the above-mentioned endonucleases or cells treated with the above-mentioned endonucleases, and therapeutic compositions obtained therefrom. |