http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016519950-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-92 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-625 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-575 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-006 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-35 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-575 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 2014-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2016519950-A |
| titleOfInvention | Method for producing peptides using engineered inteins |
| abstract | The present invention provides a method for producing peptides by recombinant means. This peptide is expressed as part of a fusion peptide comprising the target peptide and the engineered intein. The invention also provides engineered inteins, fusion proteins comprising the inteins, and DNA constructs encoding the fusion proteins. Thiol-induced cleavage of the fusion protein yields the carboxy-terminal α-thioester of the target peptide. The carboxy-terminal α-thioester can in principle react with any nucleophile, and thus this strategy allows a wide range of carboxy-terminal modifications such as chemical ligation, bioconjugation or amidation. . The engineered intein of the present invention is minimized in size and has a cysteine mutation at a position corresponding to position 3 of the Mxe GyrA intein (SEQ ID NO: 1) by alignment, thereby reducing the expression level of the fusion protein. Increased and isolated target peptides result in high yields, so the method of the present invention is suitable for production scale. |
| priorityDate | 2013-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1282.