http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016516410-A
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate | 2014-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2016516410-A |
titleOfInvention | Nucleic acid amplification method using clamp oligonucleotide |
abstract | The present invention provides a method of amplifying a target nucleic acid using a clamp oligonucleotide comprising a first target binding region on the 3′-end and a second target binding region on the 5′-end and a tether region therebetween. . The tether region can include a wide variety of user-defined sequences or elements that allow further manipulation of the target nucleic acid. For example, capture and subsequent amplification, identification and / or sequencing. The target binding region binds to the target nucleic acid, and the 3′-end serves as a primer to initiate extension along the target nucleic acid sequence, and ligation of the gap results in the formation of a circular nucleic acid. This circular template can be used for a variety of processes including amplification and sequencing. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2019532090-A |
priorityDate | 2013-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 205.