| abstract |
The present invention relates to a recombinant adenovirus having increased safety, tissue specificity and anticancer activity in vivo, and to its use, and more particularly, a liver tissue-specific PEPCK (phosphophenolate carbokinase) gene. A trans-splicing ribozyme acting on a cancer-specific gene operably linked to the promoter, a therapeutic or reporter gene linked to the ribozyme 3 ′ exon, and a serotype ) Recombinant adenovirus comprising 35 fiber knobs and a serotype 5 shaft and lacking the orf4 gene from adenovirus E1, E3 and E4 orf1 Remarkable safety in vivo, exhibits a high specificity and significant anti-cancer effect on target tissues, it can be usefully used as an anticancer agent or cancer diagnostic agent as gene transfer vectors. |