http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016220675-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b19eaa8e535da4e4bceed99917a68153 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-26 |
| filingDate | 2016-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea3b0d3cb656e162f280d88e2528b4de |
| publicationDate | 2016-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2016220675-A |
| titleOfInvention | Method, kit and apparatus for preparing sugar chains from glycoproteins |
| abstract | [Problem] To provide a method capable of rapidly carrying out a sugar chain release treatment. SOLUTION: A glycosylation enzyme is allowed to act on a sample containing a glycoprotein in the presence of a deglycosylation accelerator containing an acid-derived anionic surfactant to decompose the glycoprotein into a protein and a sugar chain. A method for releasing a sugar chain from a glycoprotein, comprising a release step of releasing the sugar chain. Thus, by using a deglycosyl chain accelerator containing an acid-derived anionic surfactant, it is possible to release a sugar chain from a glycoprotein without degrading the protein portion. A method that significantly reduces the time required for release treatment. As the acid-derived anionic surfactant, active species hidden ionic surfactants can be used, preferably carboxylic acid type anionic surfactants, more preferably N-acylamino acid type surfactants. A method for preparing a sugar chain, which is an agent and is particularly preferably an N-acyl amino acid salt or an N-acyl-N-alkyl amino acid salt. [Selection] Figure 4 |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11325935-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018062167-A1 |
| priorityDate | 2015-05-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 158.