abstract |
A thermostable glycoside hydrolase that exhibits both cellobiohydrolase activity and endoglucanase activity at least at 65 ° C, and in the presence of Ca ions at 70 ° C, and encodes the thermostable glycoside hydrolase. Provided are a polynucleotide, a vector for expressing the enzyme, a transformant incorporating the vector, and a method for producing a cellulose degradation product using the enzyme. A thermostable glycoside hydrolase having hydrolytic activity using phosphoric acid swollen Avicel (PSA) as a substrate and hydrolytic activity using carboxymethylcellulose (CMC) as a substrate under the conditions of at least 65 ° C. and pH 4.0. . A polypeptide consisting of a specific amino acid sequence or a polypeptide consisting of an amino acid sequence in which one or more of the amino acid sequences are deleted, substituted or added, etc. Thermostable glycoside hydrolase bound via [Selection figure] None |