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filingDate 2015-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2016-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2016151482-A
titleOfInvention Method for measuring electrochemical concentration of endotoxin
abstract The present invention provides a means capable of measuring the concentration of endotoxin more easily and with high sensitivity (on the order of 100 pg / mL) as compared with conventional electrochemical measurements. A method for measuring the concentration of endotoxin contained in a sample, wherein (1) the sample is brought into contact with a carrier on which a first endotoxin recognition molecule is immobilized, and the endotoxin in the sample is captured and concentrated on the carrier. (2) contacting the endotoxin recognition probe containing the heavy metal compound and the second endotoxin recognition molecule with the carrier, capturing the endotoxin recognition probe on the endotoxin on the carrier, and (3) reacting the endotoxin recognition probe with an acidic solution. And eluting heavy metal ions from the heavy metal compound of the probe, and (4) electrochemically measuring the amount of heavy metal ions eluted using a working electrode for measuring heavy metal ions. As a measure of the endotoxin concentration in the sample. [Selection] Figure 1
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022004167-A1
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