http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016131537-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efbfdc7316d3b1b7ceaee5366a61b6e0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fe9efcec7d9ed77f844ba87d886fb92c |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate | 2015-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6f6661e688ce3fd96885a41921b7e5cd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56c0c1b6def28aed7781665ee7238483 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7be094e806f8dfcc801db9123df1ccc6 |
publicationDate | 2016-07-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2016131537-A |
titleOfInvention | Method for producing cell structure into which nucleic acid is introduced |
abstract | An object of the present invention is to provide a method for producing a cell structure into which a nucleic acid is introduced with high nucleic acid introduction efficiency and in a simple manner. A preparation step for preparing a low cloud point type temperature-responsive polymer composition comprising a polymer of 2- (N, N-dimethylaminoethyl) methacrylate and / or a derivative thereof and a nucleic acid, and a low cloud point type A step of preparing a coated cell culture device by coating the culture surface of the cell culture device with a temperature-responsive polymer composition, and a cell density of 100 cells / mm 2 or more on the culture surface of the coated cell culture device A first seeding step for seeding cells, a first culture step for culturing the cells seeded in the first seeding step until a cell density of 200 cells / mm 2 or more, and a cell density of 3000 cells / mm 2 or more A nucleic acid introduced, comprising: a second seeding step of seeding on the seeded cells of the coated cell incubator; and a second culture step of culturing the cells seeded in the second seeding step. Method for producing cell structure[Selection] Figure 2 |
priorityDate | 2015-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 513.