http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016059347-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c97f90ab12b35b650d5fd039ed7a41a5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_acae886d7fc13db991247cb273eb71bd |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N37-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N37-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2014-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb483f257febbd3897b66c6277322b00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7cfe32f41fff0596518421383aef41ea http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9458d6f1ea022a50d9528bfde5157ba6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_51cf4fce50b2ee45c4ebccd8087164c0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c26f9e4e9711b1a69d644e520bff9ec |
| publicationDate | 2016-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2016059347-A |
| titleOfInvention | Method for analyzing intracellular nucleic acid, and system and kit therefor |
| abstract | [Problem] To amplify nucleic acids contained in cells in a microchamber, preparation that can be easily and reliably filled with a nucleic acid amplification reaction reagent in a short time and then sealed with a sealing solution Provide a method. A flow path device including a micro chamber chip having a micro chamber capable of containing and holding cells formed on a surface thereof and a ceiling forming member for forming a flow path having a ceiling on the top is used. A method for amplifying nucleic acids contained in cells in the microchamber, (1) a step of feeding a cell suspension into the flow path so as to accommodate the cells in the microchamber; (2) a step of sending a nucleic acid amplification reaction reagent to the channel and at least filling the microchamber with the nucleic acid amplification reaction reagent; and (3) a sealing solution being fed to the channel to fill the microchamber. A method for preparing nucleic acid amplification, comprising a step of filling the flow path with a sealing liquid so that nucleic acid amplification reaction reagents do not communicate with each other. [Selection] Figure 1 |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2017026516-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019131592-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020241689-A1 |
| priorityDate | 2014-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 199.