http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016054718-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2e85961bb2dd7bc5dc990953233d799e |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
| filingDate | 2014-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e30d5706401b5e3b3fbaa09d62147b72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f983615c679258fe4df370252f146dec |
| publicationDate | 2016-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2016054718-A |
| titleOfInvention | Methods for producing and purifying proteins and fusion proteins |
| abstract | An object of the present invention is to provide a technique that can be easily and efficiently produced without inactivating a recombinant protein. (A) A transformant comprising an expression vector comprising a gene encoding a fusion protein in which a peptide having a regulatable associating ability is added to the soluble protein at the N-terminal and / or the C-terminal thereof. Culturing the (B) recovering the transformant, crushing the cells in an aqueous solution under conditions where the peptide regions exhibit a strong associating force, and recovering a precipitate fraction containing the fusion protein; and (C) a step of suspending the precipitate fraction in an aqueous solution in which peptide regions lose their association ability or weaken their association force, extract the fusion protein, and recover the fusion protein in the soluble fraction , A method for producing a recombinant protein, comprising: [Selection figure] None |
| priorityDate | 2014-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 193.