| abstract |
A method for preparing a stably linked conjugate of a cell binding agent, such as an antibody with an effector group or reporter group, with high purity without interchain cross-linked and inactivated linker residues And provides a highly homogenous conjugate. A direct reaction of a thiol-containing cytotoxic agent (eg, maytansinoid) with a hetero- or homobifunctional reagent (eg, a cleavable or non-cleavable linker) is utilized, followed by unpurified The reaction mixture is mixed with a cell binding agent (e.g., antibody or fragment thereof), thereby being represented by the formula CL-CBA by a manufacturing process that is more efficient, has a high yield, and is suitable for scale up. To produce a non-reducing thiol bond conjugate. (C is an effector or reporter molecule; L is a linker; CBA is a cell binding agent) [Selection figure] None |