| abstract |
The present invention includes cellulase activity, endoglucanase, cellobiohydrolase, β-glucosidase, α-glucosidase, xylanase, mannanase, β-xylosidase, α-xylosidase, galactosidase, arabinofuranosidase, α-fucosidase, β-galactanase, Provided is the nucleotide sequence of Macrophomina phaseolina (“M. phaseolina”) encoding a protein / enzyme with cellulose activity, including unsaturated β-glucuronyl hydrolase and / or oligomerase activity. Also provided are vectors, expression constructs and host cells comprising and / or consisting of the nucleotide sequence of the enzyme gene. The present invention further provides methods for producing the enzymes and methods for modifying the enzymes to improve their desirable characteristics. The enzymes of the present invention can be used in various medicines, agriculture, food and feed processing, biofuels, energy efficiency and industrial relations, without limitation. These enzymes are also useful in fully hydrolyzing lignocellulosic biomass into monosaccharides that can then be fermented into liquid fuels and chemical industry raw materials. |