| abstract |
The present invention discloses an isolated polynucleotide encoding an enzyme derived from the fungus Macrophomina phaseolina (“M. phaseolina”) involved in the degradation of pectin, which is SEQ ID NO: 2, 5, 8 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44, 47, 50, 53, 56, 59, 62, 1, 4, 7, 10, 13, 16, 19 , 22, 25, 28, 31, 34, 37, 40, 43, 46, 49, 52, 55, 58 and 61, and / or the complement of such sequences Sequence or complement. The invention is also shown in SEQ ID NOs: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 60 and 63. Isolated polypeptides encoded by these polynucleotide sequences; recombinant gene constructs comprising these polynucleotides; traits comprising these recombinant gene constructs that are accompanied by or enhanced production of pectin degrading enzymes It also relates to transformants and transgenic fungi. The polypeptides of the present invention can be used for fruit juice, textile products, pulp and paper, coffee, tea production, and oil extraction and pectin drainage treatment, among others. |