http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2015524249-A
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-40 |
filingDate | 2013-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2015-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2015524249-A |
titleOfInvention | Method for producing recombinant human α-galactosidase A |
abstract | A method for the production of recombinant human α-galactosidase A (rh α-Gal A) on a large scale and with high purity is disclosed. The method consists of the following steps: (a) culturing human α-Gal A-producing mammalian cells in serum-free medium, (b) collecting the culture supernatant, and (c) collecting the culture supernatant by anion exchange column chromatography. (D) hydrophobic column chromatography, (e) column chromatography using a material having an affinity for phosphate groups as a solid phase, (f) cation exchange column chromatography, and (g ) Including gel filtration column chromatography in this order. |
priorityDate | 2012-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 159.