http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2015507928-A
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2320-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-141 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-9015 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6862 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-111 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6813 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-25 |
filingDate | 2013-02-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2015-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2015507928-A |
titleOfInvention | MIRNA analysis method |
abstract | The present invention provides a PCR-free, multiplex ligation assay for miRNA expression analysis, which produces highly quantitative, 10-100 plex miRNA profiling in a single reaction. This inventive method is a two-step ligation to generate an array of miRNA-specific ligation products that can be decoded and quantified by size discrimination methods such as gel electrophoresis or single molecule separation Use the assay. One embodiment is a low cost assay that can be performed using standard tools available in almost all molecular biology laboratories. This assay requires no more than a gel device and reader for detection. Other embodiments include the use of magnetic beads and other size exclusion devices that provide increasingly sensitive, lower sample consumption, and reduced processing steps. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11130988-B2 |
priorityDate | 2012-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 91.