| abstract |
Devices, systems, and methods are provided for the identification of various objects, particularly circulating tumor cells. In one aspect, the system includes, but is not limited to, a scanning system, an image storage system, and an analysis system. The analysis system identifies the desired object, such as a complete cell, based on various criteria, which may include cell nucleus area or volume, CD-45 negative status, and cytokeratin positive status It is preferable. Preferably, included are a slide for containing cells during the imaging step, a well including a flat bottom, a boundary of the outer edge of the well that determines the side of the well (the boundary is adjacent to the bottom surface of the well). And providing a fluid seal between them). Here, the present invention provides a single imaging well that provides a substantially monolayer object, eg, a cell. [Selection] Figure 3 |