| abstract |
A method for stably culturing human or primate embryonic stem cells (ES cells) in vitro for a long period of time is provided. A method of culturing primate or human ES cells on a positively charged particle comprising a coated matrix. The particles have a longest dimension of 50-400 μm, for example about 200 μm, and are substantially elongated, cylindrical or rod-shaped particles with a cross-sectional dimension of 20-30 μm, or about 20 μm to about 120 μm, for example about 65 μm. Of substantially compact or spherical shape. First and second primate or human ES cells attached to each particle are contacted to form a cell aggregate, and the aggregate is cultured to propagate at least one passage of the primate or human ES cell And a method of culturing ES cells in vitro by the above method under conditions that induce ES cell differentiation. [Selection] Figure 1A |