http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2015221036-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_54ae5e452ed609179e635c91ad116e6a |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-96 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2015-06-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_537cdf498a8b6efc01400eb3b7a8a9f4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9fab394c6179097dec8a0a9ff6338fcc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c075daf845c87a4675d97447cd40568 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea8eb7f6073dd8e20617c69b3917a95a |
| publicationDate | 2015-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2015221036-A |
| titleOfInvention | RNase H based assay using modified RNA monomers |
| abstract | A method for detecting a target nucleic acid sequence in a sample using a cycling probe reaction is provided. A) (i) an oligonucleotide probe having a cleavage domain lacking an RNA residue, (ii) a nucleic acid sample that may or may not have a target sequence, (iii) Providing a reaction mixture comprising a thermophilic RNase H2 enzyme capable of cleaving oligonucleotide probes when hybridized to a target nucleic acid sequence; b) probing the target DNA sequence to form a double-stranded substrate C) cleaving the hybridized probe using the RNase H2 enzyme at or within the cleavage domain or adjacent to the cleavage domain; and d) detecting probe cleavage. A method for detecting a target nucleic acid sequence comprising the step of: [Selection figure] None |
| priorityDate | 2008-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 2122.