| abstract |
PROBLEM TO BE SOLVED: To provide a multispecific peptide ligand comprising a polypeptide capable of covalently binding to a molecular scaffold at 3 or more amino acid residues and binding to 2 or more distinct targets. (A) providing a first repertoire of polypeptides, each polypeptide having two or more reactive groups capable of covalently binding to a molecular scaffold, and two reactive groups Comprising at least one loop comprising a sequence of two or more amino acids present in between, (b) providing a second repertoire of polypeptides described in (a), and (c) Linking at least one loop of one or more members of one repertoire with at least one loop of one or more members of a second repertoire to form at least one polypeptide comprising two loops; and (D) conjugating the composite polypeptide (s) to the molecular scaffold at at least three amino acid positions. [Selection figure] None |