| abstract |
The present invention provides a method capable of measuring kinase activity with high sensitivity, an ATP derivative, and a reagent kit containing the same. A step of reacting a kinase, a substrate, and an ATP derivative containing a DNP group to introduce a DNP group into the substrate, a step of mixing a DNP group-containing substrate and an anti-DNP antibody, and a formed complex A method for measuring kinase activity, wherein the ATP derivative is a compound in which a dinitrophenyl group is bound to a phosphate group at the γ-position of ATP via a linker, And a reagent kit containing the ATP derivative. [Selection] Figure 1 |