http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2015163851-A

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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
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filingDate 2014-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5051514a44022d7477dcad3c2f49e91
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publicationDate 2015-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2015163851-A
titleOfInvention Method for quantifying total protein in sample and reagent used in the method
abstract For example, in a urinalysis method for early nephropathy, not only denatured albumin and low molecular weight proteins are detected, but also a method capable of highly sensitive detection of trace amounts of proteins and uniformly detecting each protein species, Furthermore, the present invention provides a method that can be easily applied to an automatic analyzer, and a reagent for use in the method that can be used as an inexpensive and normal protein measurement reagent. A method of quantifying total protein contained in a liquid sample, wherein the sample is made alkaline and a metal is added to coordinate the metal to the protein, and the obtained sample is neutralized. And further adding at least one metal chelator selected from the group consisting of Chromazurol B, Chromazurol S, pyrogallol red, bromopyrogallol red, and 5-Br-PAPS to form a metal chelator on the metal coordinated to the protein. Chelating. [Selection] Figure 1
priorityDate 2014-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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