| abstract |
An object of the present invention is to provide a method for producing or improving a protein, particularly an antibody. The present invention relates to a gene construct in which at least an arbitrary gene and a drug resistance gene are arranged between two site-specific recombinase target sequences, and from the upstream side of the gene construct, site-specific A gene construct in which the target sequence of the target recombinase, an arbitrary gene, the drug resistance gene, and the target sequence of the site-specific target recombinase are arranged in this order, and only the drug resistance gene is placed in the reverse direction. [Selection figure] None |