| abstract |
An assay is provided for detecting and measuring the presence or level of anti-TNFα drug therapeutics and autoantibodies in a sample. A first sample from a subject is contacted with labeled TNFα to form a first complex, which is detected by size exclusion chromatography, thereby measuring the level of an anti-TNFα drug (a And a second sample from the subject is contacted with a labeled anti-TNFα drug to form a second complex of anti-TNFα drug and autoantibody, detected by size exclusion chromatography, and the level of autoantibody is determined. Subtracting the level of the autoantibody measured in step (b) from the level of the anti-TNFα drug measured in step (b), step (a) to determine the effective amount of the anti-TNFα drug Including step (c). [Selection] Figure 4 |