| abstract |
A fusion protein for protein detection having high versatility, high detection sensitivity, and high stability is provided. A protein domain containing at least one of the C1 domain of protein G, the C2 domain of protein G, and the C3 domain of protein G, and the amino acid residue Asp at position 153 of Escherichia coli alkaline alkaline phosphatase (BAP) are added by Gly. The double mutant D153G / D330N in which the amino acid residue Asp at position 330 was replaced with Asn, the amino acid residue Asp at position 153 of Escherichia coli alkaline alkaline phosphatase (BAP) was replaced with His, and the amino acid residue at position 330 The double mutant D153H / D330N in which Asp was replaced with Asn, or the amino acid residue Lys at position 328 of Escherichia coli alkaline alkaline phosphatase (BAP) was replaced with Arg, and the amino acid residue Asp at position 330 was replaced with Asn 2 A mutant K328R / D330N and protein detection fusion protein is obtained by fusing. [Selection] Figure 1 |