http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2015027283-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1347d9c8ff78439fafe12842a5b9afe3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_aaed5b1389a69a2130ed896c091cb368 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_36db5376c5dfe348c31e76aed4e2f3bf http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_440b3059c9bcd1d85fd383f9b2ae1b40 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate | 2013-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6baf72fb97b7ccac6207315a23a16065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f7ea5b536a6b4fdbb1c233a8a9f1998 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_916e580164e1153233523dc3af067dce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a22d27e27d8e132be28f28f0368a927c |
publicationDate | 2015-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2015027283-A |
titleOfInvention | Multiplex DNA typing method and primer set of cynomolgus monkey MHC gene |
abstract | The present invention provides a DNA typing method capable of selecting a cynomolgus monkey individual having a specific Mafa gene type quickly and easily. A method for DNA typing of a cynomolgus monkey Mafa gene comprising the following steps. (1) From the exon 2 region to the exon 4 region of the Mafa-A and Mafa-B genes in the cynomolgus monkey cDNA nucleotide sequence, from the exon 1 region of the Mafa-DRB, Mafa-DQA1, Mafa-DQB1, Mafa-DPA1 and Mafa-DPA1 genes Preparing a primer set for amplifying the exon 3 region under the same PCR conditions; (2) Mafa-A, Mafa-B, Mafa-DRB, Mafa-DQA1, Mafa-DQB1, Mafa-DPA1 and Mafa Amplifying by performing PCR under the same PCR conditions in one or three containers using a cDNA synthesized from a test sample (RNA) as a template using the primer set of DPB1 (where, If you use one container, All primer sets are used in the vessel, and when using three containers, the first container uses Mafa-A and Mafa-B primer sets and the second container uses Mafa-DRB primer sets. And using a primer set of Mafa-DQA1, Mafa-DQB1, Mafa-DPA1 and Mafa-DPB1 in the third container); (3) determining the base sequence of the PCR product; and (4) optionally, Performing a homology search with a database. [Selection figure] None |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112813171-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112813171-A |
priorityDate | 2013-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 56.