| abstract |
A mouse having a restricted immunoglobulin heavy chain locus, wherein the locus is characterized by a single polymorphic human VH gene segment, a plurality of human DH gene segments, and a plurality of JH gene segments. Provided. A method for generating an antibody sequence that binds to an antigen (eg, a viral antigen) comprising immunizing a mouse with an antigen of interest, wherein the mouse is a single at an endogenous immunoglobulin heavy chain locus. A method is provided comprising a single human VH gene segment, a plurality of human DH gene segments and a plurality of JH gene segments. |