| abstract |
A method for identifying reticulocytes in a blood sample disposed on a substrate, the step of irradiating the sample with incident light of two different wavelengths, the two-dimensional of the sample corresponding to the first wavelength Obtaining an image, obtaining a two-dimensional image of the sample corresponding to the second wavelength, analyzing the image to identify a representative set of red blood cells, each of the red blood cells in the set Determining the area; determining the brightness of each of the red blood cells in the set; and for each red blood cell in the set, the area of the red blood cell exceeds the area cutoff value And a step of identifying a red blood cell as a reticulocyte when the lightness of the red blood cell is smaller than a lightness cutoff value. |