abstract |
Use a primary clarified depth filtration device of feed material, including chemically treated, soft agglomerated feed material, containing biomolecules of interest, eg, mAb, mammalian cell culture or bacterial cell culture And a method for primary clarification without using a primary clarification centrifugation step or a primary clarification tangential flow microfiltration step. The primary clarified depth filtration device contains a porous depth filter having graded porous layers of various pore grades. The primary clarified depth filtration device is a chemically treated, soft agglomerated feed containing soft agglomerated cell debris and colloidal microparticles having a particle size distribution of about 0.5 μm to about 200 μm The fluid feed material containing the material is filtered at a flow rate from 10 liters / m 2 / hour to about 100 liters / m 2 / hour. Kits and their use and fabrication are also provided. |