abstract |
In microbial fermentation, it is desirable to increase the protein product yield. This is because, in addition to the first expression construct encoding the protein, an auxiliary protease comprising an amino acid sequence that is proteolytically active and at least 50% identical to the amino acid sequence set forth in SEQ ID NO: 1, unlike the protein This is achieved by a method of introducing a second expression construct encoding the above into a microorganism. |