| abstract |
A transaminase biocatalyst is provided. The present disclosure relates to a polypeptide having transaminase activity, a polynucleotide encoding the polypeptide, and a method of using the polypeptide. In another aspect, the present disclosure provides a host cell comprising a polynucleotide encoding an engineered transaminase, or an expression vector capable of expressing the engineered transaminase. In some embodiments, the host cell is E. coli. Bacterial host cells such as E. coli can be used. Host cells can be used to express and isolate the engineered transaminase enzymes described herein, or alternatively they can be used directly to convert ketoamide substrates to products. can do. [Selection figure] None |