| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_966a6c1f382ec9bcb5e3839dfd73e5b8 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2549-119
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-55
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-143
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6881
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-005
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-08 |
| filingDate |
2014-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2369bd199159e744607ccd816a628872
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4e1f2662ee9fdf70a435a32a6823161d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d7c9e3663102e06947a7ba388448e8ff
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7983a45ed84f69c3b65cdd2b854e178b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_59f503d71c8b56ca823e4e59950410d5 |
| publicationDate |
2014-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2014221069-A |
| titleOfInvention |
Methods for obtaining nucleic acids encoding immunoglobulins |
| abstract |
The present invention provides a method for obtaining a nucleic acid encoding an immunoglobulin variable domain from a single immunoglobulin-producing cell. A first polymerase chain reaction using 3 to 6 5 ′ primers and one 3 ′ primer, and 13 to 16 5 ′ primers and one 3 ′ primer are used. A method comprising a step of performing a second polymerase chain reaction with a product of the first polymerase chain reaction, wherein a distance between binding positions of primers used in the second polymerase chain reaction is reduced as compared with the first polymerase chain reaction. [Selection figure] None |
| priorityDate |
2009-02-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |