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filingDate 2013-04-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6f3d713c360acd8ae1f716b2dbbbe178
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publicationDate 2014-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2014212719-A
titleOfInvention Microbiological testing method
abstract PROBLEM TO BE SOLVED: To easily and quickly detect dominant species of microorganisms in a test for confirming the presence or absence of microorganisms in the environment. SOLUTION: A step of collecting a sample from the environment, culturing microorganisms contained in the obtained sample in a medium, and two or more colonies formed by culturing the microorganisms are formed. Dividing a colony into two or more sets, extracting a nucleic acid of a microorganism contained in the set for each set, amplifying a nucleic acid fragment including an amplification target region using the extracted nucleic acid, and an amplified nucleic acid fragment And detecting the microorganisms included in the set for each set, and when two or more types of microorganisms are detected in the microorganism detecting step, the total number of the two or more sets is detected for each detected microorganism. By calculating the ratio of the number of aggregates in which each microorganism was detected to the number of aggregates, the microorganism testing method includes a step of determining the dominant species of microorganisms in the environment. [Selection figure] None
priorityDate 2013-04-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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