| abstract |
The present invention provides an scFv antibody that passes through an epithelial and / or endothelial layer. From a pool of antibody frameworks that specifically bind to a selected antigen, and (i) a soluble and stable antibody framework, optimally for a framework of non-human antibodies that have a specific binding specificity for the antigen Selecting a matching, soluble and stable framework; (ii) providing said soluble and stable framework with a CDR that specifically binds to said antigen, or framework of said non-human antibody Are mutated into the soluble and stable framework sequences described above to generate scFv antibodies, (iii) the generated antibodies are tested for solubility and stability, and the antibodies generated And (iv) selecting a scFV that is soluble, stable and specifically binds to the antigen. Obtainable by law, scFv antibodies. [Selection figure] None |