http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2014164096-A

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publicationDate 2014-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2014164096-A
titleOfInvention Scanning observation device
abstract A high-resolution fluorescence image is obtained while suppressing an afterimage even when a fluorescence detection interval is shortened. A scanning unit that spatially scans a specimen with pulsed excitation light generated at a predetermined time interval from a light source, and the inside of the specimen A by excitation light scanned by the scanning part. The fluorescence detection unit 4 that detects the fluorescence generated by the excitation of the fluorescent substance in synchronization with the generation of the excitation light, and the fluorescence intensity detected by the fluorescence detection unit 4 at each scanning position, before that. Provided is a scanning observation apparatus 1 including a fluorescence correction unit 5 that subtracts an afterimage fluorescence component calculated based on time-series fluorescence detected by the fluorescence detection unit 4 to correct fluorescence intensity at the scanning position. To do. [Selection] Figure 1
isCitedBy http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-7032817-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016197045-A
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