| abstract |
PROBLEM TO BE SOLVED: To provide an overcharged protein for cell penetration. The present invention provides a composition, preparation for delivering a supercharged protein or a complex of a supercharged protein and an agent (eg, nucleic acid, peptide, protein, small molecule) to a cell. Articles, systems and related methods are provided. Such systems and methods involve the use of overcharged proteins. For example, an excessively positively charged protein can be associated with a nucleic acid (typically having a net negative charge) by electrostatic interaction. In some embodiments, such systems and methods involve modifying the primary sequence of the protein to “overcharge” the protein (eg, to produce a protein that is overly positively charged). Including. In some embodiments, a complex comprising a supercharged protein and one or more agents to be delivered is useful as a therapeutic agent. [Selection] Figure 6 |