abstract |
Disclosed are a cassette containing a promoter of a target gene and a gene manipulation method using the cassette. A promoter-specific homologous site that is homologous to at least a part of a promoter of a target gene, a marker gene operably linked to the promoter-specific homologous site, and a target gene linked downstream of the marker gene The present invention relates to a cassette for deleting a target gene containing a gene-specific homologous site that is homologous to at least a part of the gene, and a gene manipulation method using the cassette. By using the cassette and a genetic manipulation method using the cassette, efficient genetic manipulation and selection of cells are possible. [Selection] Figure 1B |