abstract |
The substrate specificity of a lipolytic enzyme is determined by altering the amino acid sequence of a given region of the lipolytic enzyme so as to increase the level of desired activity or decrease the level of undesirable activity. Can be changed. Thus, we aim to develop a lipolytic enzyme variant with a modified amino acid sequence that has substrate specificity that can be tailored for a particular application. The present invention relates to a method for altering the substrate specificity of a lipolytic enzyme by modifying the amino acid sequence, and to a lipolytic enzyme mutant obtained by such modification. The present invention also relates to a method for screening a lipolytic enzyme. [Selection figure] None |