http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2014138558-A

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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071
filingDate 2013-01-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c202100f16efaa4cd71ae07e753d4c6b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5769d19aa36e2b968709f223a7dc8e74
publicationDate 2014-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2014138558-A
titleOfInvention Method for producing sheet cell culture
abstract 【Task】 Provided is a method for obtaining a relatively large size, clinically safe and high-quality sheet-like cell culture. [Solution] (I) seeding the cells on the substrate, (ii) pressurizing the cells against the substrate, (iii) culturing the cells to form a sheet-like cell culture, and (iv) A step of releasing the formed culture from the substrate, wherein the repeated set consisting of steps (i) and (ii) is repeated two or more times, sowing and / or pressurization in the second and subsequent repeated sets The above-mentioned problem has been solved by a method for producing a sheet-shaped cell culture, which is carried out so as to correct the density of the cell density due to the bias in the pressurization after the end of the immediately preceding pressurization step. [Selection figure] None
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