http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2014108073-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_639cdb1f1acfa83266e034f782aeb15b |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2012-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93d48ab00697fabdeb5fbea833b0b353 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db93888ecb374a984577a0eab318781b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_363abe1486d4110c8719db15cfbe19a0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0c36578f48240ee953e7bc2bd0d82980 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19d92865b83488a616b30af7860c561f |
| publicationDate | 2014-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2014108073-A |
| titleOfInvention | Method for preparing recombinant human pancreatic lipase |
| abstract | The present invention provides a method for preparing a large amount of recombinant human pancreatic lipase (recHPL) having specific activity close to that of natural porcine pancreatic lipase. (A) a step of constructing an expression plasmid obtained by adding Strep-tagII to the C-terminal side of HPL cDNA; (b) introducing the constructed expression plasmid into Escherichia coli, culturing, and then introducing HPL into E. coli. (C) a step of purifying HPL in one step using sepharose carrying streptactin from an E. coli lysate obtained by disrupting the E. coli; and (d) urea purified by adding Ca 2+ to the purified HPL. The refolding step is activated using a glycerol step dialysis method in which the concentration is lowered stepwise. [Selection figure] None |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-101813703-B1 |
| priorityDate | 2012-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 182.