http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2014094936-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6bfdf49224a405be1f7b653c56ebad94 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 |
filingDate | 2013-10-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_473ff0e71c6ef3c96a9660b6f1c2d1b1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d065f2ea4415a361f65b422d7db758ae |
publicationDate | 2014-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2014094936-A |
titleOfInvention | Antibody purification method |
abstract | Provided is a method for purifying the antibody with high purity by efficiently removing impurities contained in the solution when the antibody is purified from the solution containing the antibody using affinity chromatography. A step of adding an antibody-containing solution to an equilibrated column packed with a carrier for affinity chromatography, a first washing step of washing with the solution used for equilibration of the column, and a final concentration of 0.2M. A purification method comprising a second washing step of washing with a neutral or basic buffer containing sodium chloride and a step of washing impurities contained in the solution containing the antibody. Alternatively, the step of adding sodium chloride so as to be 0.2 M or more to the solution containing the antibody, the step of adding the solution containing the antibody to the column, and the solution containing the antibody in the solution used for equilibration of the column A purification method comprising a step of washing impurities contained therein and a step of eluting the antibody using an eluate. [Selection] Figure 6 |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114409765-A |
priorityDate | 2012-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 63.