http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2014087334-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0669f10ac82c082c17a3bf9d4a941b96 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-02 |
| filingDate | 2013-10-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c87d6c483f49a0e307bef3422c67a89c |
| publicationDate | 2014-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2014087334-A |
| titleOfInvention | High-efficiency production method of specific antibodies enabling rapid diagnosis of microorganisms |
| abstract | A method for producing a specific antibody capable of rapid diagnosis of microorganisms with high efficiency is provided. A method for producing a hybridoma comprising the following steps: 1) an immunization step in which a microorganism-derived antigen is repeatedly administered to an organism and an antigen-specific receptor is expressed on the B cell surface; 2) the B cell surface A B cell-biotinylated antigen complex is formed by binding an antigen-specific receptor expressed in to a biotinylated antigen and then streptavidin is bound to form a B cell-biotinylated antigen-streptavidin complex. Steps (1), 3) Binding myeloma cells and biotin to produce biotinylated myeloma cells, 4) Cell cross-linking step to obtain B cells-myeloma cells by cross-linking by biotin-avidin reaction, 5) B cells A hybridoma production method comprising: a hybridoma production step of obtaining a hybridoma by applying a DC rectangular wave electric pulse to a myeloma cell. Made. [Selection] Figure 3 |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112626063-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112626063-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021193750-A1 |
| priorityDate | 2012-10-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 262.