http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2014082936-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_716e9a2606ffd4ba1a2a3917e4a402fd |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-52 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2012-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_761c92b7822afe2ff3345407d37c5881 |
publicationDate | 2014-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2014082936-A |
titleOfInvention | Mutant reverse transcriptase |
abstract | [PROBLEMS] To provide a mutant reverse transcriptase capable of performing a reverse transcription reaction efficiently even under a temperature condition that is excellent in industrial productivity and suppresses the formation of secondary structure, and a nucleic acid encoding the same, and a mutant reverse Provided are a method for producing a transcriptase, a reverse transcription method, a reverse transcription reaction kit and a detection kit. In the amino acid sequence of wild-type MMLV reverse transcriptase, an amino acid residue corresponding to at least one residue selected from the group consisting of F303, L432, V433 and I434 was substituted with a positively charged amino acid residue. Mutant reverse transcriptase, nucleic acid encoding the same, method for producing mutant reverse transcriptase for recovering mutant reverse transcriptase from a culture obtained by culturing cells retaining the nucleic acid, mutant reverse transcriptase A reverse transcription method for synthesizing cDNA from RNA using RNA, a reverse transcription reaction kit containing the mutant reverse transcriptase, and a detection kit containing the mutant reverse transcriptase and a marker detection reagent. [Selection figure] None |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10793922-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11505836-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2016063797-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113174381-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2021045140-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2017536813-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113174381-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-7114676-B2 |
priorityDate | 2012-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 119.