abstract |
A method for rapidly amplifying one or more loci in a nucleic acid sample, a thermal cycler, and a system useful for performing the method are provided. A high-speed multiplex PCR system capable of rapidly generating an amplified nucleic acid product (eg, a complete STR profile) from a target nucleic acid. Multiplex PCR system with microfluidic biochip and special thermal cycler. The resulting STR profile provides forensic guidelines for signal strength, peak height balance at internal loci, peak height ratio in heterozygotes, addition of incomplete nucleotides without templates, and stutter. It is possible to satisfy. [Selection figure] None |