| abstract |
The present invention generally relates to a reference data set or “scorecard” for pluripotent stem cells, and creates a scorecard for predicting the functionality and suitability of a pluripotent stem cell line for a desired use. The present invention relates to a method, a system and a kit. In some aspects, the method of creating a scorecard is selected from epigenetic profiling, differentiation assays, and gene expression assays to predict the functionality and suitability of pluripotent stem cell lines for the desired use Using at least two stem cell assays. In some embodiments, scorecard reference data can be compared with pluripotent stem cell line data to effectively and accurately predict the usefulness of pluripotent stem cells for a given application, and for example, therapeutic use Can identify specific characteristics of pluripotent stem cell lines for determining its suitability for downstream applications, such as its suitability for differentiation, drug screening, and toxicity assays, and desired cell lineages . |