| abstract |
Systems, devices, kits, and methods for purification and analysis of analytes having a wide range of hydrophobicity by liquid chromatography-mass spectrometry (LC-MS). Using a set of liquid chromatography columns, a set of mobile phase buffers, and optionally a single ionization method (eg, electrospray ionization), a wide range of analytes can be analyzed by liquid chromatography-mass spectrometry (LC-MS ) System can be purified and analyzed. LC-MS purification and analysis of analytes with a wide range of partition coefficients can be achieved by changing column or buffer or LC-MS by selecting special LC operating parameters and MS system parameters for different classes of analytes. This is accomplished without having to make any other hardware configuration changes to the system. The methods, systems, and kits described herein provide a wide range of analytes without compromising inherent specificity for individual analytes compared to previously reported methods. Provides substantially increased speed / throughput and ease of use. |